Journal: Immunobiology

Article Title: c-Rel is dispensable for the differentiation and functional maturation of M cells in the follicle-associated epithelium

doi: 10.1016/j.imbio.2016.09.008

Figure Lengend Snippet: Primers used for RT-qPCR analysis.

Article Snippet: For the detection of SpiB in paraformaldehyde-fixed sections, antigen retrieval was performed with citrate buffer (pH 7.0, 121 °C, 5 min) prior to immunostaining with sheep anti-mouse SpiB polyclonal antibody (R&D Systems).

Techniques:

Journal: Immunobiology

Article Title: c-Rel is dispensable for the differentiation and functional maturation of M cells in the follicle-associated epithelium

doi: 10.1016/j.imbio.2016.09.008

Figure Lengend Snippet: SpiB expression in the FAE is unaffected in the absence of c-Rel. (A) IHC analysis of SpiB expression (green) in the FAE of c-Rel −/− and wild-type (WT) control mice. Boxed areas in upper panels are shown at higher magnification in the lower panels. Broken lines indicate the FAE boundaries. Arrows, Spi-B + cell nuclei in the FAE. (B) Morphometric analysis showed that the number of SpiB + cells in the FAE of c-Rel −/− and WT were similar. (C) RT-qPCR analysis suggested there was no significant difference in the expression of Spib mRNA levels in Peyer’s patches from c-Rel −/− or WT mice. Gene expression data are normalised so that the mean level in samples from WT mice was 1.0. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: For the detection of SpiB in paraformaldehyde-fixed sections, antigen retrieval was performed with citrate buffer (pH 7.0, 121 °C, 5 min) prior to immunostaining with sheep anti-mouse SpiB polyclonal antibody (R&D Systems).

Techniques: Expressing, Control, Quantitative RT-PCR, Gene Expression

Journal: eLife

Article Title: POMK regulates dystroglycan function via LARGE1-mediated elongation of matriglycan

doi: 10.7554/eLife.61388

Figure Lengend Snippet:

Article Snippet: Antibody , anti-sheep IgG (Donkey polyclonal) , Rockland , Cat# 613-731-168, RRID: AB_220181 , WB (1:2000).

Techniques: Sequencing, Recombinant, Control, Purification, Plasmid Preparation, Software, Western Blot, Isolation

Journal: Macromolecular bioscience

Article Title: Patterned hydrogel substrates for cell culture with electrohydrodynamic jet printing.

doi: 10.1002/mabi.201100004

Figure Lengend Snippet: Figure 3. Droplets of IgG printed on 9.6 kPa (a) and 34.4 kPa (b) gels. Scale bars are 50 mm. Confocal image shows that IgG was located at the surface, but penetrated approximately 30 mm into a 21.1 kPa gel (c). Immunofluorescent detection of printed fibronectin on 21.1 kPa gels showing precise placement with spot sizes down to approximately 5 mm in diameter (d–f). Scale bars are 50 mm.

Article Snippet: Fibronectin was visualized after treatment with sheep anti-human fibronectin IgG (R&D Systems) and NL493-conjugated donkey anti-sheep IgG (R&D Systems).

Techniques:

Journal: Macromolecular bioscience

Article Title: Patterned hydrogel substrates for cell culture with electrohydrodynamic jet printing.

doi: 10.1002/mabi.201100004

Figure Lengend Snippet: Figure 4. A primary mesenchymal stem cell stained for filamen- tous actin (a) elongated and aligned with a fibronectin stripe (b) detected with immunofluorescence. A composite image is shown in (c). A phase contrast image of D1 cells adhered to equally spaced spots of fibronectin (d). Spots can be printed with a small enough diameter to constrain single cells. A phase contrast image of densely packed D1s on a narrow rectangle of patterned fibro- nectin (e). Scale bars are 50 mm.

Article Snippet: Fibronectin was visualized after treatment with sheep anti-human fibronectin IgG (R&D Systems) and NL493-conjugated donkey anti-sheep IgG (R&D Systems).

Techniques: Staining

Journal: Scientific Reports

Article Title: Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells

doi: 10.1038/srep35680

Figure Lengend Snippet: ( a ) Schematic outline of endothelial cell differentiation. ( b ) Purity of PSC-EPCs on day 7. Data are presented as the mean ± SEM (n = 3) and were statistically analyzed using ANOVA test. ( c ) Representative flow cytometry plots of cells (day 7) on LM411 (KhES-1). ( d ) Tube formation assay of PSC-ECs induced on LM411. Scale bar: 200 μm. ( e ) Ac-LDL-uptake and CD31 expression of PSC-ECs induced on LM411. Scale bar: 10 μm.

Article Snippet: Anti-human KDR antibodies (BioLegend), anti-human CD34 antibodies (Beckman coulter), anti-human VE-cadherin antibodies (eBioscience), sheep anti-human CD31 antibodies (R&D systems), FITC-conjugated anti-sheep IgG antibodies (Jackson ImmunoResearch), mouse anti-human nuclei antibodies (Millipore), Cy3-conjugated anti-mouse IgG antibodies (Jackson ImmunoResearch), Alexa-Fluor 647-labelled rat anti-mouse TER-119 antibodies (BioLegend), rat anti-human CD49f antibodies (GoH3, BD), anti-human CD29 antibodies (AIIB2; developed by Caroline H. Damsky and obtained from Developmental Studies Hybridoma Bank) were used in this study.

Techniques: Cell Differentiation, Flow Cytometry, Tube Formation Assay, Expressing

Journal: Scientific Reports

Article Title: Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells

doi: 10.1038/srep35680

Figure Lengend Snippet: ( a ) Schematic process of the modified differentiation system via activation of the canonical Wnt signaling pathway. ( b ) Representative flow cytometry plots of the day 6 cell population (KhES-1). ( c ) Efficiency of endothelial differentiation in the modified system compared to the unmodified system. The ordinates indicate the number of PSC-EPCs per starting PSC number. Data are presented as the mean ± SEM (n = 3) and were statistically analyzed using Student’s t -test. Representative results of at least three independent experiments are shown. ( d ) The cell numbers on day 3 (undefined system) and day 2 (defined system), normalized by starting PSC numbers. The data were analyzed using Student’s t -test, n = 3. ( e ) CD34 + VE-cadherin + cell numbers at day 6. Cell numbers were normalized by plated cell numbers on LM411-E8. The data were analyzed using Student’s t -test, n = 3. ( f ) A tube formation assay of PSC-ECs. Scale bars: 200 μm. ( g ) Ac-LDL-uptake and CD31 expression of PSC-ECs. Scale bar: 10 μm. ( h ) Expression of vWF, eNOS and PECAM1 in PSC-EPCs compared to undifferentiated (day 0) cells. The RQ values were calculated via the ΔΔCt-method using GAPDH on day 0 as an internal control. ( i ) ICAM-1 expression in PSC-ECs stimulated with 10 ng/mL TNF-α for 18 hours. ( j ) The proportion of CD34 + VE-Cadherin + cells on LM411-E8 or LM511-E8. Day 2 cells were plated cultured for four days. The data were analyzed using Student’s t -test, n = 3. ( k ) Immunofluorescence images of Matrigel-plug sections. PSC-EPCs (KhES-1) generated according to the above method were suspended in Matrigel and subcutaneously transplanted to NOG mice. Human endothelial cells (positive for HuNu and CD31) can be seen surrounding murine erythrocytes (positive for TER-119). Scale bars: 5 μm.

Article Snippet: Anti-human KDR antibodies (BioLegend), anti-human CD34 antibodies (Beckman coulter), anti-human VE-cadherin antibodies (eBioscience), sheep anti-human CD31 antibodies (R&D systems), FITC-conjugated anti-sheep IgG antibodies (Jackson ImmunoResearch), mouse anti-human nuclei antibodies (Millipore), Cy3-conjugated anti-mouse IgG antibodies (Jackson ImmunoResearch), Alexa-Fluor 647-labelled rat anti-mouse TER-119 antibodies (BioLegend), rat anti-human CD49f antibodies (GoH3, BD), anti-human CD29 antibodies (AIIB2; developed by Caroline H. Damsky and obtained from Developmental Studies Hybridoma Bank) were used in this study.

Techniques: Modification, Activation Assay, Flow Cytometry, Tube Formation Assay, Expressing, Control, Cell Culture, Immunofluorescence, Generated